Journal: MedComm

Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes

doi: 10.1002/mco2.70437

Figure Lengend Snippet: Genes differentially expressed in the dormant metastasis group. Heat maps are presented to illustrate the differential expression data between the three metastatic groups obtained from RNA‐Seq (A), or RT‐qPCR in genes involved in the cholesterol synthesis pathway (B), in chemokine genes (C), and in surface marker genes (D). Only genes that displayed a greater than twofold difference in expression (log2>1) with a p ‐value less than 0.001 were identified and plotted in the heatmap. The expression values are presented as log2 values and are scaled by gene. In RT‐qPCR assays, the expression levels of the genes of interest were determined with respect to the levels of the β‐actin and GAPDH housekeeping genes. The data for the overt‐met group were set to 1. The values represent the means of three independent experiments performed in duplicate. The statistical analysis was performed using ANOVA, followed by Tukey's post hoc test.

Article Snippet: The miProfileMouse miRNome miRNA qPCR Array (catalog QM002; GeneCopoeia) was used to analyze 834 mouse miRNAs.

Techniques: Quantitative Proteomics, RNA Sequencing, Quantitative RT-PCR, Marker, Expressing

Journal: MedComm

Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes

doi: 10.1002/mco2.70437

Figure Lengend Snippet: MicroRNAs that are differentially expressed in the dormant metastasis group. (A) Heat map is presented to illustrate the differential expression data between the three metastatic groups. (B) miRNA enrichment analysis of miRNAs differentially expressed in dormant‐met group (miEAA). The expression of 834 miRNAs was measured using the ‘mouse miRNome qPCR arrays 18.0’ (Genecopoeia). Only miRNAs that displayed a greater than twofold difference ( p < 0.01) when comparing the dormant‐met group with the nude‐met and overt‐met groups were plotted. The expression levels of the miRNAs of interest were determined with respect to the levels of six housekeeping snRNAs (MK1‐MK6). The data for the overt‐met group were set to 1. The values are presented as the means of three independent experiments, each performed in duplicate. The statistical analysis was conducted using an ANOVA test, followed by a Tukey's post hoc test.

Article Snippet: The miProfileMouse miRNome miRNA qPCR Array (catalog QM002; GeneCopoeia) was used to analyze 834 mouse miRNAs.

Techniques: Quantitative Proteomics, Expressing

Journal: Nucleic Acids Research

Article Title: Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation

doi: 10.1093/nar/gkaf1290

Figure Lengend Snippet: Dynamic regulation of biosynthesis pathway with tryptophan-responsive MR-scRNAs. ( A ) Design schematic illustrating the dynamic regulatory strategy used to conditionally activate the violacein biosynthesis pathway in response to concentrations of the upstream metabolite, tryptophan, that is required for both growth and biochemical production. ( B ) Dose–response curve of the tryptophan MR-scRNA in response to the target metabolite. ( C ) Violacein titer extracted from E. coli cells with the violacein biosynthesis pathway activated by an on-target scRNA (+), non-targeting scRNA (−), or a tryptophan-responsive scRNA (MR-scRNA). Abbreviations: Ø, not detected; a.u., arbitrary units.

Article Snippet: Violacein biosynthesis genes were obtained from Addgene plasmid 73440 (a gift from Mattheos Koffas) [ ].

Techniques: